2eho
From Proteopedia
Crystal structure of human GINS complex
Contents |
Overview
The GINS complex mediates the assembly of the MCM2-7 (minichromosome maintenance) complex with proteins in a replisome progression complex. The eukaryotic GINS complex is composed of Sld5, Psf1, Psf2, and Psf3, which must be assembled for cell proliferation. We determined the crystal structure of the human GINS complex: GINS forms an elliptical shape with a small central channel. The structures of Sld5 and Psf2 resemble those of Psf1 and Psf3, respectively. In addition, the N-terminal and C-terminal domains of Sld5/Psf1 are permuted in Psf2/Psf3, which suggests that the four proteins have evolved from a common ancestor. Using a structure-based mutational analysis, we identified the functionally critical surface regions of the GINS complex.
Disease
Known disease associated with this structure: Bare lymphocyte syndrome, type I, due to TAP2 deficiency OMIM:[170261], Wegener-like granulomatosis OMIM:[170261]
About this Structure
2EHO is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the human GINS complex., Choi JM, Lim HS, Kim JJ, Song OK, Cho Y, Genes Dev. 2007 Jun 1;21(11):1316-21. PMID:17545466 Page seeded by OCA on Sun May 4 02:34:11 2008
