2ejy

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Template:STRUCTURE 2ejy

Solution structure of the p55 PDZ T85C domain complexed with the glycophorin C F127C peptide

Overview

p55, a member of the membrane-associated guanylate kinase family, includes a PDZ domain that specifically interacts with the C-terminal region of glycophorin C in the ternary complex of p55, protein 4.1 and glycophorin C. Here we present the first NMR-derived complex structure of the p55 PDZ domain and the C-terminal peptide of glycophorin C, obtained by using a threonine to cysteine (T85C) mutant of the p55 PDZ domain and a phenylalanine to cysteine (F127C) mutant of the glycophorin C peptide. Our NMR results revealed that the two designed mutant molecules retain the specific interaction manner that exists between the wild type molecules and can facilitate the structure determination by NMR, due to the stable complex formation via an intermolecular disulfide bond. The complex structure provides insight into the specific interaction of the p55 PDZ domain with the two key residues, Ile128 and Tyr126, of glycophorin C.

About this Structure

2EJY is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural insight into the interaction between the p55 PDZ domain and glycophorin C., Kusunoki H, Kohno T, Biochem Biophys Res Commun. 2007 Aug 10;359(4):972-8. Epub 2007 Jun 11. PMID:17572384 Page seeded by OCA on Sun May 4 02:39:37 2008