1wbj
From Proteopedia
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WILDTYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH GLYCEROL PHOSPHATE
Overview
Indole is a reaction intermediate in at least two biosynthetic pathways in, maize seedlings. In the primary metabolism, the alpha-subunit (TSA) of the, bifunctional tryptophan synthase (TRPS) catalyzes the cleavage of indole, 3-glycerol phosphate (IGP) to indole and d-glyceraldehyde 3-phosphate, (G3P). Subsequently, indole diffuses through the connecting tunnel to the, beta-active site where it is condensed with serine to form tryptophan and, water. The maize enzyme, BX1, a homolog of TSA, also cleaves IGP to G3P, and indole, and the indole is further converted to, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant, metabolite. BX1 cleaves IGP significantly faster to G3P and indole than, does TSA. In line with their different biological functions, these two, ... [(full description)]
About this Structure
1WBJ is a [Protein complex] structure of sequences from [Salmonella typhimurium] with NA, G3P and PLP as [ligands]. Active as [[1]], with EC number [4.2.1.20]. Full crystallographic information is available from [OCA].
Reference
On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and BX1 from maize, two evolutionarily related enzymes., Kulik V, Hartmann E, Weyand M, Frey M, Gierl A, Niks D, Dunn MF, Schlichting I, J Mol Biol. 2005 Sep 23;352(3):608-20. PMID:16120446
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