2i9a

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spinqualitylabelsall models 2i9a, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the free aminoterminal fragment of urokinase type plasminogen activator (ATF)

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Recent studies indicate that binding of the urokinase-type plasminogen, activator (uPA) to its high-affinity receptor (uPAR) orchestrates uPAR, interactions with other cellular components that play a pivotal role in, diverse (patho-)physiological processes, including wound healing, angiogenesis, inflammation, and cancer metastasis. However, notwithstanding the wealth of biochemical data available describing the, activities of uPAR, little is known about the exact mode of uPAR/uPA, interactions or the presumed conformational changes that accompany, uPA/uPAR engagement. Here, we report the crystal structure of soluble, urokinase plasminogen activator receptor (suPAR), which contains the three, domains of the wild-type receptor but lacks the cell-surface anchoring, sequence, in complex with the amino-terminal fragment of urokinase-type, plasminogen activator (ATF), at the resolution of 2.8 A. We report the 1.9, A crystal structure of free ATF. Our results provide a structural basis, represented by conformational changes induced in uPAR, for several, published biochemical observations describing the nature of uPAR/uPA, interactions and provide insight into mechanisms that may be responsible, for the cellular responses induced by uPA binding.

Disease

Known disease associated with this structure: Alzheimer disease, late-onset, susceptibility to OMIM:[191840]

About this Structure

2I9A is a Single protein structure of sequence from Homo sapiens with PO4 as ligand. Active as U-plasminogen activator, with EC number 3.4.21.73 Full crystallographic information is available from OCA.

Reference

Structural basis of interaction between urokinase-type plasminogen activator and its receptor., Barinka C, Parry G, Callahan J, Shaw DE, Kuo A, Bdeir K, Cines DB, Mazar A, Lubkowski J, J Mol Biol. 2006 Oct 20;363(2):482-95. Epub 2006 Aug 26. PMID:16979660

Page seeded by OCA on Mon Nov 12 22:42:33 2007