2idh

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spinqualitylabelsall models 2idh, resolution 2.28Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of human FE65 WW domain

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The neuronal protein FE65 functions in brain development and amyloid, precursor protein (APP) signaling through its interaction with the, mammalian enabled (Mena) protein and APP, respectively. The recognition of, short polyproline sequences in Mena by the FE65 WW domain has a central, role in axon guidance and neuronal positioning in the developing brain. We, have determined the crystal structures of the human FE65 WW domain, (residues 253-289) in the apo form and bound to the peptides PPPPPPLPP and, PPPPPPPPPL, which correspond to human Mena residues 313-321 and 347-356, respectively. The FE65 WW domain contains two parallel ligand-binding, grooves, XP (formed by residues Y269 and W280) and XP2 (formed by Y269 and, W271). Both Mena peptides adopt a polyproline helical II conformation and, bind to the WW domain in a forward (N-C) orientation through selection of, the PPPPP motif by the XP and XP2 grooves. This mode of ligand recognition, is strikingly similar to polyproline interaction with SH3 domains., Importantly, comparison of the FE65 WW structures in the apo and liganded, forms shows that the XP2 groove is formed by an induced-fit mechanism that, involves movements of the W271 and Y269 side-chains upon ligand binding., These structures elucidate the molecular determinants underlying, polyproline ligand selection by the FE65 WW domain and provide a framework, for the design of small molecules that would interfere with FE65 WW-ligand, interaction and modulate neuronal development and APP signaling.

Disease

Known disease associated with this structure: Alzheimer disease, late-onset OMIM:[602710]

About this Structure

2IDH is a Single protein structure of sequence from Homo sapiens with SO4 and PG4 as ligands. Full crystallographic information is available from OCA.

Reference

Structural Basis for Polyproline Recognition by the FE65 WW Domain., Meiyappan M, Birrane G, Ladias JA, J Mol Biol. 2007 Sep 28;372(4):970-80. Epub 2007 Jun 29. PMID:17686488

Page seeded by OCA on Mon Nov 12 22:43:46 2007