2o94

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spinqualitylabelsall models 2o94, resolution 3.000Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The 97H/F mutant Structure of a glutamine-rich domain from histone deacetylase 4

Overview

Glutamine-rich sequences exist in a wide range of proteins across multiple, species. A subset of glutamine-rich sequences has been shown to form, amyloid fibers implicated in human diseases. The physiological functions, of these sequence motifs are not well understood, partly because of the, lack of structural information. Here we have determined a high-resolution, structure of a glutamine-rich domain from human histone deacetylase 4, (HDAC4) by x-ray crystallography. The glutamine-rich domain of HDAC4 (19, glutamines of 68 residues) folds into a straight alpha-helix that, assembles as a tetramer. In contrast to most coiled coil proteins, the, HDAC4 tetramer lacks regularly arranged apolar residues and an extended, hydrophobic core. Instead, the protein interfaces consist of multiple, hydrophobic patches interspersed with polar interaction networks, wherein, clusters of glutamines engage in extensive intra- and interhelical, interactions. In solution, the HDAC4 tetramer undergoes rapid equilibrium, with monomer and intermediate species. Structure-guided mutations that, expand or disrupt hydrophobic patches drive the equilibrium toward the, tetramer or monomer, respectively. We propose that a general role of, glutamine-rich motifs be to mediate protein-protein interactions, characteristic of a large component of polar interaction networks that may, facilitate reversible assembly and disassembly of protein complexes.

About this Structure

2O94 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of a conserved N-terminal domain of histone deacetylase 4 reveals functional insights into glutamine-rich domains., Guo L, Han A, Bates DL, Cao J, Chen L, Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4297-302. Epub 2007 Mar 5. PMID:17360518

Page seeded by OCA on Mon Nov 12 23:07:55 2007