We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2gjc

From Proteopedia

Revision as of 02:10, 4 May 2008 by OCA (Talk | contribs)
Jump to: navigation, search
Image:2gjc.gif

Template:STRUCTURE 2gjc

Crystal Structure of Thiazole Synthase in Saccharomyces cerevisiae at 1.82 A resolution


Overview

The structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae was determined to 1.8 A resolution. Thi4 exists as an octamer with two monomers in the asymmetric unit. The structure reveals the presence of a tightly bound adenosine diphospho-5-(beta-ethyl)-4-methylthiazole-2-carboxylic acid at the active site. The isolation of this reaction product identifies NAD as the most likely precursor and provides the first mechanistic insights into the biosynthesis of the thiamin thiazole in eukaryotes. Additionally, the Thi4 structure reveals the first protein structure with a GR(2) domain that binds NAD instead of FAD, raising interesting questions about how this protein evolved from a flavoenzyme to a NAD binding enzyme.

About this Structure

2GJC is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae., Jurgenson CT, Chatterjee A, Begley TP, Ealick SE, Biochemistry. 2006 Sep 19;45(37):11061-70. PMID:16964967 Page seeded by OCA on Sun May 4 05:10:23 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gjc"
Personal tools