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2hdc
From Proteopedia
STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX
Overview
Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.
About this Structure
2HDC is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex., Jin C, Marsden I, Chen X, Liao X, J Mol Biol. 1999 Jun 18;289(4):683-90. PMID:10369754 Page seeded by OCA on Sun May 4 06:08:55 2008
Categories: Rattus norvegicus | Single protein | Chen, X. | Jin, C. | Liao, X. | Marsden, I. | Dyanamic | Genesis | Nmr | Structure | Winged helix protein
