2joo
From Proteopedia
The NMR Solution Structure of Recombinant RGD-hirudin
Overview
The solution structure of a new recombinant RGD-hirudin, which has the activities of anti-thrombin and anti-platelet aggregation, was determined by (1)H nuclear magnetic resonance spectroscopy and compared with the conformations of recombinant wild-type hirudin and hirudin (variant 2, Lys47) of the hirudin thrombin complex. On the basis of total 1284 distance and dihedral angle constraints derived from a series of NMR spectra, 20 conformers were computed with ARIA/CNS programs. The structure of residues 3-30 and 37-48 form a molecular core with two antiparallel beta-sheets as the other two hirudins. However, significant differences were found in the surface electrostatic charge distributions among the three hirudins, especially in the RGD segment of recombinant RGD-hirudin. This difference may be greatly beneficial to its additional function of anti-platelet aggregation. The difference in extended C-terminal makes its both ionic and hydrophobic interactions with the fibrinogen recognition exosite of thrombin more effective.
About this Structure
2JOO is a Single protein structure of sequence from Hirudo medicinalis. Full crystallographic information is available from OCA.
Reference
The NMR solution structure of recombinant RGD-hirudin., Song X, Mo W, Liu X, Zhu L, Yan X, Song H, Dai L, Biochem Biophys Res Commun. 2007 Aug 17;360(1):103-8. Epub 2007 Jun 13. PMID:17585879 Page seeded by OCA on Sun May 4 09:07:23 2008
Categories: Hirudo medicinalis | Single protein | Dai, L. | Liu, X. | Mo, W. | Song, H. | Song, X. | Yan, X. | Blood clotting | Mainly beta
