Acid-beta-glucosidase
From Proteopedia
Acid-beta-glucosidase
Acid-beta-glucosidase is a lysozomal enzyme, which cleaves glucoceramide to glucose and ceramide. It acts through an acid-base hydrolysis.
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According to the , an enzyme, which acts through the acid-base reacion has to contain in the active site a proton donor, an acid, and a nuclophile, the base. The nuclophile is involved in formation and stabilization of the intermediate state, were a proton is transferred from an anomeric carbon to the leaving group. At that stage, the proton donor would donate its proton to the intermediate complex, releasing the second product and hence acting as a catalytic acid. Such a mechanism depends strongly on the pH of the environment. Fine changes in acidity of the solution might affect the ionization states of the residues involved in the catalysis. Therefore there would be a strong dependence of the enzyme's activity on the pH of the solution.
Several x-ray structures of the enzyme have been solved. PDB codes: 1y7v; 1ogs; 2f61; 2nt0; 2nt1; 2v3d; 2v3e; 2nsx; 2j25
Gaucher disease
Gaucher disease, the most common lysosomal storage disorder (LSD), is caused by mutations in the gene encoding acid-β-glucosidase (enzyme classification E.C. 3.2.1.45), resulting in intracellular accumulation of glucosylceramide (GlcCer).
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Boris Brumshtein, Alexander Berchansky, Joel L. Sussman, Eran Hodis, David Canner
