2o61
From Proteopedia
Crystal Structure of NFkB, IRF7, IRF3 bound to the interferon-b enhancer
Overview
Transcriptional activation of the interferon-beta (IFN-beta) gene requires assembly of an enhanceosome containing ATF-2/c-Jun, IRF-3/IRF-7, and NFkappaB. These factors bind cooperatively to the IFN-beta enhancer and recruit coactivators and chromatin-remodeling proteins to the IFN-beta promoter. We describe here a crystal structure of the DNA-binding domains of IRF-3, IRF-7, and NFkappaB, bound to one half of the enhancer, and use a previously described structure of the remaining half to assemble a complete picture of enhanceosome architecture in the vicinity of the DNA. Association of eight proteins with the enhancer creates a continuous surface for recognizing a composite DNA-binding element. Paucity of local protein-protein contacts suggests that cooperative occupancy of the enhancer comes from both binding-induced changes in DNA conformation and interactions with additional components such as CBP. Contacts with virtually every nucleotide pair account for the evolutionary invariance of the enhancer sequence.
About this Structure
2O61 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
An atomic model of the interferon-beta enhanceosome., Panne D, Maniatis T, Harrison SC, Cell. 2007 Jun 15;129(6):1111-23. PMID:17574024 Page seeded by OCA on Sun May 4 10:22:23 2008
