1fj1
From Proteopedia
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LYME DISEASE ANTIGEN OSPA IN COMPLEX WITH NEUTRALIZING ANTIBODY FAB LA-2
Overview
Outer surface protein A (OspA) is a major lipoprotein of the Borrelia, burgdorferi spirochete, the causative agent of Lyme disease. Vaccination, with OspA generates an immune response that can prevent bacterial, transmission to a mammalian host during the attachment of an infected, tick. However, the protective capacity of immune sera cannot be predicted, by measuring total anti-OspA antibody. The murine monoclonal antibody LA-2, defines an important protective B-cell epitope of OspA against which, protective sera have strong levels of reactivity. We have now mapped the, LA-2 epitope of OspA using both NMR chemical-shift perturbation, measurements in solution and X-ray crystal structure determination. LA-2, recognizes the three surface-exposed loops of the C-terminal domain of, OspA that are on the tip of the elongated molecule most distant from the, lipid-modified N terminus. The structure suggests that the natural, variation at OspA sequence position 208 in the first loop is a major, limiting factor for antibody cross-reactivity between different Lyme, disease-causing Borrelia strains. The unusual Fab-dominated lattice of the, crystal also permits a rare view of antigen flexibility within an, antigen:antibody complex. These results provide a rationale for, improvements in OspA-based vaccines and suggest possible designs for more, direct tests of antibody protective levels in vaccinated individuals.
About this Structure
1FJ1 is a Single protein structure of sequence from Borrelia burgdorferi and Mus musculus. This structure superseeds the now removed PDB entry 2OSP. Full crystallographic information is available from OCA.
Reference
Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA., Ding W, Huang X, Yang X, Dunn JJ, Luft BJ, Koide S, Lawson CL, J Mol Biol. 2000 Oct 6;302(5):1153-64. PMID:11183781
Page seeded by OCA on Sun Nov 18 09:30:18 2007
