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1a0a
From Proteopedia
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PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX
Overview
The crystal structure of a DNA-binding domain of PHO4 complexed with DNA, at 2.8 A resolution revealed that the domain folds into a, basic-helix-loop-helix (bHLH) motif with a long but compact loop that, contains a short alpha-helical segment. This helical structure positions a, tryptophan residue into an aromatic cluster so as to make the loop, compact. PHO4 binds to DNA as a homodimer with direct reading of both the, core E-box sequence CACGTG and its 3'-flanking bases. The 3'-flanking, bases GG are recognized by Arg2 and His5. The residues involved in the, E-box recognition are His5, Glu9 and Arg13, as already reported for, bHLH/Zip proteins MAX and USF, and are different from those recognized by, bHLH proteins MyoD and E47, although PHO4 is a bHLH protein.
About this Structure
1A0A is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition., Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, Oshima Y, Hakoshima T, EMBO J. 1997 Aug 1;16(15):4689-97. PMID:9303313
Page seeded by OCA on Tue Nov 20 10:31:47 2007
