1ajw
From Proteopedia
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STRUCTURE OF RHOGDI: A C-TERMINAL BINDING DOMAIN TARGETS AN N-TERMINAL INHIBITORY PEPTIDE TO GTPASES, NMR, 20 STRUCTURES
Overview
The Rho GDP-dissociation inhibitors (GDIs) negatively regulate Rho-family, GTPases. The inhibitory activity of GDI derives both from an ability to, bind the carboxy-terminal isoprene of Rho family members and extract them, from membranes, and from inhibition of GTPase cycling between the GTP- and, GDP-bound states. Here we demonstrate that these binding and inhibitory, functions of rhoGDI can be attributed to two structurally distinct regions, of the protein. A carboxy-terminal folded domain of relative molecular, mass 16,000 (M[r] 16K) binds strongly to the Rho-family member Cdc42, yet, has little effect on the rate of nucleotide dissociation from the GTPase., The solution structure of this domain shows a beta-sandwich motif with a, narrow hydrophobic cleft that binds isoprenes, and an exposed surface that, interacts with the protein portion of Cdc42. The amino-terminal region of, rhoGDI is unstructured in the absence of target and contributes little to, binding, but is necessary to inhibit nucleotide dissociation from Cdc42., These results lead to a model of rhoGDI function in which the, carboxy-terminal binding domain targets the amino-terminal inhibitory, region to GTPases, resulting in membrane extraction and inhibition of, nucleotide cycling.
About this Structure
1AJW is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
C-terminal binding domain of Rho GDP-dissociation inhibitor directs N-terminal inhibitory peptide to GTPases., Gosser YQ, Nomanbhoy TK, Aghazadeh B, Manor D, Combs C, Cerione RA, Rosen MK, Nature. 1997 Jun 19;387(6635):814-9. PMID:9194563
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