1b3c
From Proteopedia
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SOLUTION STRUCTURE OF A BETA-NEUROTOXIN FROM THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING
Overview
We report the detailed solution structure of the 7.2 kDa protein CsE-I, a, beta-neurotoxin from the New World scorpion Centruroides sculpturatus, Ewing. This toxin binds to sodium channels, but unlike the, alpha-neurotoxins, shifts the voltage of activation toward more negative, potentials causing the membrane to fire spontaneously. Sequence-specific, proton NMR assignments were made using 600 MHz 2D-NMR data. Distance, geometry and dynamical simulated annealing refinements were performed, using experimental distance and torsion angle constraints from NOESY and, pH-COSY data. A family of 40 structures without constraint violations was, generated, and an energy-minimized average structure was computed. The, backbone conformation of the CsE-I toxin shows similar secondary, structural features as the prototypical alpha-neurotoxin, CsE-v3, and is, characterized by a short 2(1/2)-turn alpha-helix and a 3-strand, antiparallel beta-sheet, both held together by disulfide bridges. The RMSD, for the backbone atoms between CsE-I and CsE-v3 is 1.48 A. Despite this, similarity in the overall backbone folding, the these two proteins show, some important differences in the primary structure (sequence) and, electrostatic potential surfaces. Our studies provide a basis for, unravelling the role of these differences in relation to the known, differences in the receptor sites on the voltage sensitive sodium channel, for the alpha- and beta-neurotoxins.
About this Structure
1B3C is a Single protein structure of sequence from Centruroides sculpturatus. Full crystallographic information is available from OCA.
Reference
Solution structure of a beta-neurotoxin from the New World scorpion Centruroides sculpturatus Ewing., Jablonsky MJ, Jackson PL, Trent JO, Watt DD, Krishna NR, Biochem Biophys Res Commun. 1999 Jan 19;254(2):406-12. PMID:9918851
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