1akw
From Proteopedia
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G61L OXIDIZED FLAVODOXIN MUTANT
Overview
Mutants of the electron-transfer protein flavodoxin from Desulfovibrio, vulgaris were made by site-directed mutagenesis to investigate the role of, glycine-61 in stabilizing the semiquinone of FMN by the protein and in, controlling the flavin redox potentials. The spectroscopic properties, oxidation-reduction potentials, and flavin-binding properties of the, mutant proteins, G61A/N/V and L, were compared with those of wild-type, flavodoxin. The affinities of all of the mutant apoproteins for FMN and, riboflavin were less than that of the wild-type apoprotein, and the redox, potentials of the two 1-electron steps in the reduction of the complex, with FMN were also affected by the mutations. Values for the dissociation, constants of the complexes of the apoprotein with the semiquinone and, ... [(full description)]
About this Structure
1AKW is a [Single protein] structure of sequence from [Desulfovibrio vulgaris] with FMN as [ligand]. Full crystallographic information is available from [OCA].
Reference
Modulation of the redox potentials of FMN in Desulfovibrio vulgaris flavodoxin: thermodynamic properties and crystal structures of glycine-61 mutants., O'Farrell PA, Walsh MA, McCarthy AA, Higgins TM, Voordouw G, Mayhew SG, Biochemistry. 1998 Jun 9;37(23):8405-16. PMID:9622492
Page seeded by OCA on Mon Oct 29 19:25:43 2007
