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spinqualitylabelsall models 1bex, resolution 2.3Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF RUTHENIUM-MODIFIED PSEUDOMONAS AERUGINOSA AZURIN

Overview

The crystal structure of Ru(2, 2'-bipyridine)2(imidazole)(His83)azurin, (RuAz) has been determined to 2.3 A -resolution by X-ray crystallography., The spectroscopic and thermodynamic properties of both the native protein, and [Ru(2, 2'-bipyridine)2(imidazole)2]2+ are maintained in the modified, protein. Dark-green RuAz crystals grown from PEG 4000, LiNO3, CuCl2 and, Tris buffer are monoclinic, belong to the space group C2 and have cell, parameters a = 100.6, b = 35.4, c = 74.7 A and beta = 106. 5 degrees. In, addition, [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O was synthesized, crystallized and structurally characterized by X-ray crystallography., Red-brown crystals of this complex are monoclinic, space group P21/n, unit-cell parameters a = 13.230 (2), b = 18.197 (4), c = 16.126 (4) A, beta = 108.65 (2) degrees. Stereochemical parameters for the refinement of, Ru(2, 2'-bipyridine)2(imidazole)(His83) were taken from the atomic, coordinates of [Ru(2,2'-bipyridine)2(imidazole)2]2+. The structure of RuAz, confirms that His83 is the only site of chemical modification and that the, native azurin structure is not perturbed significantly by the ruthenium, label.

About this Structure

1BEX is a Single protein structure of sequence from Pseudomonas aeruginosa with CU and RBU as ligands. Full crystallographic information is available from OCA.

Reference

Structures of ruthenium-modified Pseudomonas aeruginosa azurin and [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O., Faham S, Day MW, Connick WB, Crane BR, Di Bilio AJ, Schaefer WP, Rees DC, Gray HB, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):379-85. PMID:10089343

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