1blq
From Proteopedia
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STRUCTURE AND INTERACTION SITE OF THE REGULATORY DOMAIN OF TROPONIN-C WHEN COMPLEXED WITH THE 96-148 REGION OF TROPONIN-I, NMR, 29 STRUCTURES
Overview
The structure of the regulatory domain of chicken skeletal troponin-C, (residues 1-90) when complexed with the major inhibitory region (residues, 96-148) of chicken skeletal troponin-I was determined using multinuclear, multidimensional NMR spectroscopy. This complex represents the first, interaction formed between the regulatory domain of troponin-C and, troponin-I after calcium binding in the regulation of muscle contraction., The stoichiometry of the complex was determined to be 1:1, with a, dissociation constant in the 1-40 microM range. The structure of, troponin-C in the complex was calculated from 1039 NMR distance and 111, dihedral angle restraints. When compared to the structure of this domain, in the calcium saturated "open" form but in the absence of troponin-I, the, bound structure appears to be slightly more "closed". The troponin-I, peptide-binding site was found to be in the hydrophobic pocket of calcium, saturated troponin-C, using edited/filtered NMR experiments and chemical, shift mapping of changes induced in the regulatory domain upon peptide, binding. The troponin-I peptide (residues 96-148) was found to bind to the, regulatory domain of troponin-C very similarly, but not identically, to a, shorter troponin-I peptide (region 115-131) thought to represent the major, interaction site of troponin-I for this domain of troponin-C.
About this Structure
1BLQ is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Structure and interaction site of the regulatory domain of troponin-C when complexed with the 96-148 region of troponin-I., McKay RT, Pearlstone JR, Corson DC, Gagne SM, Smillie LB, Sykes BD, Biochemistry. 1998 Sep 8;37(36):12419-30. PMID:9730814
Page seeded by OCA on Tue Nov 20 11:44:51 2007
