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1bvs
From Proteopedia
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RUVA COMPLEXED TO A HOLLIDAY JUNCTION.
Overview
Holliday junctions occur as intermediates in homologous recombination and, DNA repair. In bacteria, resolution of Holliday junctions is accomplished, by the RuvABC system, consisting of a junction-specific helicase complex, RuvAB, which promotes branch migration, and a junction-specific, endonuclease RuvC, which nicks two strands. The crystal structure of a, complex between the RuvA protein of M. leprae and a synthetic four-way, junction has now been determined. Rather than binding on the open surface, of a RuvA tetramer as previously suggested, the DNA is sandwiched between, two RuvA tetramers, which form a closed octameric shell, stabilized by a, conserved tetramer-tetramer interface. Interactions between the DNA, backbone and helix-hairpin-helix motifs from both tetramers suggest a, mechanism for strand separation promoted by RuvA.
About this Structure
1BVS is a Single protein structure of sequence from Mycobacterium leprae. Full crystallographic information is available from OCA.
Reference
Crystal structure of an octameric RuvA-Holliday junction complex., Roe SM, Barlow T, Brown T, Oram M, Keeley A, Tsaneva IR, Pearl LH, Mol Cell. 1998 Sep;2(3):361-72. PMID:9774974
Page seeded by OCA on Tue Nov 20 11:57:33 2007
