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1ca5
From Proteopedia
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INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA
Overview
Sso7d and Sac7d are two small chromatin proteins from the, hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus, acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45, A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property, of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed, structural analysis revealed that the intercalation site includes the T-G, mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a, manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new, inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order, bridging water molecules are found. The results suggest that the less, stable DNA stacking site involving a T-G mismatch may be a preferred site, for protein side-chain intercalation.
About this Structure
1CA5 is a Single protein structure of sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA.
Reference
Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116
Page seeded by OCA on Tue Nov 20 12:18:01 2007
