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3cl3
From Proteopedia
Crystal Structure of a vFLIP-IKKgamma complex: Insights into viral activation of the IKK signalosome
Overview
Key to the pathogenicity of several viruses is activation of the canonical nuclear factor-kappaB (NF-kappaB) transcriptional pathway. Subversion of this tightly regulated mechanism is achieved through the production of host mimetic viral proteins that deregulate the transcription process. One such protein is ks-vFLIP (produced by the Kaposi's sarcoma herpes virus [KSHV]), which associates with IKKgamma, an essential component of the IKK complex or signalosome. This interaction renders the canonical NF-kappaB pathway constitutively active and has been linked to Kaposi's sarcoma and other malignancies. In order to elucidate the molecular basis underpinning ks-vFLIP-induced activation of the IKK signalosome, we have determined the crystal structure of a complex involving a fragment of IKKgamma bound to ks-vFLIP at 3.2 A. In addition to identifying and subsequently probing the ks-vFLIP-IKKgamma interface, we have also investigated the effects of a mutation implicated in the genetic disorder anhydrotic ectodermal dysplasia with immunodeficiency (EDA-ID).
About this Structure
3CL3 is a Protein complex structure of sequences from Homo sapiens and Human herpesvirus 8. Full crystallographic information is available from OCA.
Reference
Crystal structure of a vFlip-IKKgamma complex: insights into viral activation of the IKK signalosome., Bagneris C, Ageichik AV, Cronin N, Wallace B, Collins M, Boshoff C, Waksman G, Barrett T, Mol Cell. 2008 Jun 6;30(5):620-31. PMID:18538660 Page seeded by OCA on Wed Jun 18 12:10:46 2008
Categories: Homo sapiens | Human herpesvirus 8 | Protein complex | Ageichik, A V. | Bagneris, C. | Barrett, T. | Boshoff, C. | Cronin, N. | Waksman, G. | Coiled coil | Coiled-coil | Cytoplasm | Death effector domain | Disease mutation | Ectodermal dysplasia | Host-virus interaction | Nucleus | Transcription | Transcription regulation | Viral protein/signaling protein complex
