3d91

From Proteopedia

proteopedia linkproteopedia link

Template:STRUCTURE 3d91

Human renin in complex with remikiren

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Human renin has been expressed in Sf9 and CHO cells using two different gene constructs. The first construct contained a foreign signal peptide fused directly to the sequence encoding mature renin, whereas the second construct harbors the sequence for preprorenin. Prorenin was produced in significantly higher amounts than the mature enzyme expressed without its propeptide in both expression systems. Both directly expressed mature renin and proteolytically derived active renin have been purified and cocrystallized with the renin inhibitor Ro 42-5892. The 3D structure has been solved for both versions and demonstrates identity despite different glycosylation and different N termini.

Disease

Known disease associated with this structure: Renal tubular dysgenesis OMIM:[179820], Hyperproreninemia OMIM:[179820]

About this Structure

3D91 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Recombinant human renin produced in different expression systems: biochemical properties and 3D structure., Mathews S, Dobeli H, Pruschy M, Bosser R, D'Arcy A, Oefner C, Zulauf M, Gentz R, Breu V, Matile H, Schlaeger J, Fischli W, Protein Expr Purif. 1996 Feb;7(1):81-91. PMID:9172787 Page seeded by OCA on Wed Jun 18 12:13:09 2008