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1coo

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1coo

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THE COOH-TERMINAL DOMAIN OF RNA POLYMERASE ALPHA SUBUNIT

Overview

The structure of the carboxyl-terminal domain of the Escherichia coli RNA, polymerase alpha subunit (alpha CTD), which is regarded as the contact, site for transcription activator proteins and for the promoter UP element, was determined by nuclear magnetic resonance spectroscopy. Its compact, structure of four helices and two long arms enclosing its hydrophobic core, shows a folding topology distinct from those of other DNA-binding, proteins. The UP element binding site was found on the surface comprising, helix 1, the amino-terminal end of helix 4, and the preceding loop., Mutation experiments indicated that the contact sites for transcription, activator proteins are also on the same surface.

About this Structure

1COO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Solution structure of the activator contact domain of the RNA polymerase alpha subunit., Jeon YH, Negishi T, Shirakawa M, Yamazaki T, Fujita N, Ishihama A, Kyogoku Y, Science. 1995 Dec 1;270(5241):1495-7. PMID:7491496

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