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1cov
From Proteopedia
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COXSACKIEVIRUS B3 COAT PROTEIN
Overview
The crystal structure of coxsackievirus B3 (CVB3) has been determined to, 3.5 A resolution. The icosahedral CVB3 particles crystallize in the, monoclinic space group, P2(1), (a = 574.6, b = 302.1, c = 521.6 A, beta =, 107.7 degrees ) with two virions in the asymmetric unit giving 120-fold, non-crystallographic redundancy. The crystals diffracted to 2.7 A, resolution and the X-ray data set was 55% complete to 3.0,4, resolution., Systematically weak reflections and the self-rotation function established, pseudo R32 symmetry with each particle sitting on a 32 special position., This constrained the orientation and position of each particle in the, monoclinic cell to near face-centered positions and allowed for a total of, six possible monoclinic space-group settings. Correct interpretation of, the high-resolution (3.0-3.2 A) self-rotation function was instrumental in, determining the deviations from R32 orientations of the virus particles in, the unit cell. Accurate particle orientations permitted the correct, assignment of the crystal space-group setting amongst the six ambiguous, possibilities and for the correct determination of particle positions., Real-space electron-density averaging and phase refinement, using human, rhinovius 14 (HRV14) as an initial phasing model, have been carried out to, 3.5 A resolution. The initial structural model has been built and refined, to 3.5 A resolution using X-PLOR.
About this Structure
1COV is a Single protein structure of sequence from Human coxsackievirus b1 with MYR and PLM as ligands. Full crystallographic information is available from OCA.
Reference
Structure determination of coxsackievirus B3 to 3.5 A resolution., Muckelbauer JK, Kremer M, Minor I, Tong L, Zlotnick A, Johnson JE, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):871-87. PMID:15299757
Page seeded by OCA on Tue Nov 20 12:38:06 2007
