1d3c
From Proteopedia
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MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN
Overview
The enzyme cyclodextrin glycosyltransferase is closely related to, alpha-amylases but has the unique ability to produce cyclodextrins, (circular alpha(1-->4)-linked glucoses) from starch. To characterize this, specificity we determined a 1.8-A structure of an E257Q/D229N mutant, cyclodextrin glycosyltransferase in complex with its product, gamma-cyclodextrin, which reveals for the first time how cyclodextrin is, competently bound. Across subsites -2, -1, and +1, the cyclodextrin ring, binds in a twisted mode similar to linear sugars, giving rise to, deformation of its circular symmetry. At subsites -3 and +2, the, cyclodextrin binds in a manner different from linear sugars. Sequence, comparisons and site-directed mutagenesis experiments support the, conclusion that subsites -3 and +2 confer the cyclization activity in, addition to subsite -6 and Tyr-195. On this basis, a role of the, individual residues during the cyclization reaction cycle is proposed.
About this Structure
1D3C is a Single protein structure of sequence from Bacillus circulans with CA and MPD as ligands. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.
Reference
The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution., Uitdehaag JC, Kalk KH, van Der Veen BA, Dijkhuizen L, Dijkstra BW, J Biol Chem. 1999 Dec 3;274(49):34868-76. PMID:10574960
Page seeded by OCA on Tue Nov 20 12:58:57 2007
Categories: Bacillus circulans | Cyclomaltodextrin glucanotransferase | Single protein | Dijkhuizen, L. | Dijkstra, B.W. | Kalk, K.H. | Uitdehaag, J.C.M. | Veen, B.A.van.der. | CA | MPD | Alpha-amylase | Catalysis | Family 13 glycosyl hydrolase | Induced fit | Oligosaccharide | Product complex | Transglycosylation
