1d3y
From Proteopedia
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STRUCTURE OF THE DNA TOPOISOMERASE VI A SUBUNIT
Overview
In all organisms, type II DNA topoisomerases are essential for untangling, chromosomal DNA. We have determined the structure of the DNA-binding core, of the Methanococcus jannaschii DNA topoisomerase VI A subunit at 2.0 A, resolution. The overall structure of this subunit is unique, demonstrating, that archaeal type II enzymes are distinct from other type II, topoisomerases. However, the core structure contains a pair of domains, that are also found in type IA and classic type II topoisomerases., Together, these regions may form the basis of a DNA cleavage mechanism, shared among these enzymes. The core A subunit is a dimer that contains a, deep groove that spans both protomers. The dimer architecture suggests, that DNA is bound in the groove, across the A subunit interface, and that, the two monomers separate during DNA transport. The A subunit of, topoisomerase VI is homologous to the meiotic recombination factor, Spo11, and this structure can serve as a template for probing Spo11 function in, eukaryotes.
About this Structure
1D3Y is a Single protein structure of sequence from Methanocaldococcus jannaschii with NA and MG as ligands. Active as DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 Full crystallographic information is available from OCA.
Reference
Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11., Nichols MD, DeAngelis K, Keck JL, Berger JM, EMBO J. 1999 Nov 1;18(21):6177-88. PMID:10545127
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