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1di2
From Proteopedia
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CRYSTAL STRUCTURE OF A DSRNA-BINDING DOMAIN COMPLEXED WITH DSRNA: MOLECULAR BASIS OF DOUBLE-STRANDED RNA-PROTEIN INTERACTIONS
Overview
Protein interactions with double-stranded RNA (dsRNA) are critical for, many cell processes; however, in contrast to protein-dsDNA interactions, surprisingly little is known about the molecular basis of protein-dsRNA, interactions. A large and diverse class of proteins that bind dsRNA do so, by utilizing an approximately 70 amino acid motif referred to as the, dsRNA-binding domain (dsRBD). We have determined a 1.9 A resolution, crystal structure of the second dsRBD of Xenopus laevis RNA-binding, protein A complexed with dsRNA. The structure shows that the protein spans, 16 bp of dsRNA, interacting with two successive minor grooves and across, the intervening major groove on one face of a primarily A-form RNA helix., The nature of these interactions explains dsRBD specificity for dsRNA, (over ssRNA or dsDNA) and the apparent lack of sequence specificity., Interestingly, the dsRBD fold resembles a portion of the conserved core, structure of a family of polynucleotidyl transferases that includes RuvC, MuA transposase, retroviral integrase and RNase H. Structural comparisons, of the dsRBD-dsRNA complex and models proposed for polynucleotidyl, transferase-nucleic acid complexes suggest that similarities in nucleic, acid binding also exist between these families of proteins.
About this Structure
1DI2 is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA., Ryter JM, Schultz SC, EMBO J. 1998 Dec 15;17(24):7505-13. PMID:9857205
Page seeded by OCA on Tue Nov 20 13:17:43 2007
