This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1dts
From Proteopedia
|
CRYSTAL STRUCTURE OF AN ATP DEPENDENT CARBOXYLASE, DETHIOBIOTIN SYNTHASE, AT 1.65 ANGSTROMS RESOLUTION
Overview
BACKGROUND: In Escherichia coli, the enzymes of the biotin biosynthesis, pathway are encoded by the bio operon. One of these enzymes, ATP-dependent, dethiobiotin synthetase, catalyzes the carboxylation of, 7,8-diaminopelargonic acid leading to the formation of the ureido ring of, biotin. The enzyme belongs to the class of ATP-dependent carboxylases and, we present here the first crystal structure determined for this class of, enzyme. RESULTS: We have determined the crystal structure of homodimeric, dethiobiotin synthetase to 1.65 A resolution. The subunit consists of a, seven-stranded parallel beta-sheet, surrounded by alpha-helices. The sheet, contains the classical mononucleotide-binding motif with a fingerprint, peptide Gly-X-X-X-X-X-Gly-Lys-Thr. The mononucleotide binding part of the, structure is very similar to the GTP-binding protein H-ras-p21 and thus, all GTP-binding proteins. A comparison reveals that some of the residues, which in H-ras-p21 interact with the nucleotide and the metal ion, are, conserved in the synthetase. CONCLUSIONS: The three-dimensional structure, of dethiobiotin synthetase has revealed that ATP-dependent carboxylases, contain the classical mononucleotide-binding fold. Considerable, similarities to the structure of the GTP-binding protein H-ras-p21 were, found, indicating that both proteins might have evolved from a common, ancestral mononucleotide-binding fold.
About this Structure
1DTS is a Single protein structure of sequence from Escherichia coli. Active as Dethiobiotin synthase, with EC number 6.3.3.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of an ATP-dependent carboxylase, dethiobiotin synthetase, at 1.65 A resolution., Huang W, Lindqvist Y, Schneider G, Gibson KJ, Flint D, Lorimer G, Structure. 1994 May 15;2(5):407-14. PMID:8081756
Page seeded by OCA on Tue Nov 20 13:34:23 2007
