1efz
From Proteopedia
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MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY
Overview
The tRNA modifying enzyme tRNA-guanine transglycosylase (TGT) is involved, in the exchange of guanine in the first position of the anticodon with, preQ1 as part of the biosynthesis of the hypermodified base queuine (Q)., Mutation of Ser90 to an alanine in Escherichia coli TGT leads to a, dramatic reduction of enzymatic activity (Reuter, K. et al. (1994), Biochemistry 33, 7041-7046). To further clarify the role of this residue, in the catalytic center, we have mutated the corresponding Ser103 of the, crystallizable Zymomonas mobilis TGT into alanine. The crystal structure, of a TGT(S103A)/preQ1 complex combined with biochemical data presented in, this paper suggest that Ser103 is essential for substrate orientation in, the TGT reaction.
About this Structure
1EFZ is a Single protein structure of sequence from Zymomonas mobilis with ZN and PRF as ligands. Active as Queuine tRNA-ribosyltransferase, with EC number 2.4.2.29 Full crystallographic information is available from OCA.
Reference
Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity., Gradler U, Ficner R, Garcia GA, Stubbs MT, Klebe G, Reuter K, FEBS Lett. 1999 Jul 2;454(1-2):142-6. PMID:10413112
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