1ehk

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spinqualitylabelsall models 1ehk, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF THE ABERRANT BA3-CYTOCHROME-C OXIDASE FROM THERMUS THERMOPHILUS

Overview

Cytochrome c oxidase is a respiratory enzyme catalysing the, energy-conserving reduction of molecular oxygen to water. The crystal, structure of the ba(3)-cytochrome c oxidase from Thermus thermophilus has, been determined to 2.4 A resolution using multiple anomalous dispersion, (MAD) phasing and led to the discovery of a novel subunit IIa. A, structure-based sequence alignment of this phylogenetically very distant, oxidase with the other structurally known cytochrome oxidases leads to the, identification of sequence motifs and residues that seem to be, indispensable for the function of the haem copper oxidases, e.g. a new, electron transfer pathway leading directly from Cu(A) to Cu(B). Specific, features of the ba(3)-oxidase include an extended oxygen input channel, which leads directly to the active site, the presence of only one oxygen, atom (O(2-), OH(-) or H(2)O) as bridging ligand at the active site and the, mainly hydrophobic character of the interactions that stabilize the, electron transfer complex between this oxidase and its substrate, cytochrome c. New aspects of the proton pumping mechanism could be, identified.

About this Structure

1EHK is a Protein complex structure of sequences from Thermus thermophilus with BNG, CU, HEM, HAS and CUA as ligands. Active as Cytochrome-c oxidase, with EC number 1.9.3.1 Full crystallographic information is available from OCA.

Reference

Structure and mechanism of the aberrant ba(3)-cytochrome c oxidase from thermus thermophilus., Soulimane T, Buse G, Bourenkov GP, Bartunik HD, Huber R, Than ME, EMBO J. 2000 Apr 17;19(8):1766-76. PMID:10775261

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