1eqk
From Proteopedia
|
SOLUTION STRUCTURE OF ORYZACYSTATIN-I, A CYSTEINE PROTEINASE INHIBITOR OF THE RICE, ORYZA SATIVA L. JAPONICA
Overview
The three-dimensional structure of oryzacystatin-I, a cysteine proteinase, inhibitor of the rice, Oryza sativa L. japonica, has been determined in, solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy., The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and, consists of an alpha-helix and a five-stranded antiparallel beta-sheet, while the N- and C-terminal regions (Ser2-Val12 and Ala98-Ala102) are less, defined. The helix-sheet architechture of oryzacystatin-I is stabilized by, a hydrophobic cluster formed between the alpha-helix and the beta-sheet, and is considerably similar to that of monellin, a sweet-tasting protein, from an African berry, as well as those of the animal cystatins studied, e.g., chicken egg white cystatin and human stefins A and B (also referred, to as human cystatins A and B). Detailed structural comparison indicates, that oryzacystatin-I is more similar to chicken cystatin, which belongs to, the type-2 animal cystatins, than to human stefins A and B, which belong, to the type-1 animal cystatins, despite different loop length.
About this Structure
1EQK is a Single protein structure of sequence from Oryza sativa japonica group. Full crystallographic information is available from OCA.
Reference
Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica., Nagata K, Kudo N, Abe K, Arai S, Tanokura M, Biochemistry. 2000 Dec 5;39(48):14753-60. PMID:11101290
Page seeded by OCA on Tue Nov 20 14:13:55 2007
