1lde

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Image:1lde.gif

1lde, resolution 2.5Å

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HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED TO NADH AND N-FORMYL PIPERDINE

Overview

Amides are analogs of aldehydes and potent inhibitors of liver alcohol, dehydrogenases. They can be used for structural studies and for inhibiting, the metabolism of alcohols that form toxic products. We studied N-alkyl, amides that bind to the enzyme-NADH complex and act as uncompetitive, inhibitors against varied concentrations of ethanol (millimolar Kii, values, at pH 8 and 25 degrees C): N-propylacetamide (16), delta-valerolactam (1.6), N-formylpiperidine (0.14), N-isobutylformamide, (0.028), N-(cyclohexylmethyl)-formamide (0.011), and N-cyclohexylformamide, (0.0087). The lower affinity of delta-valerolactam and N-propylacetamide, can be explained by steric hindrance with Phe93 of the enzyme. Replacing, Phe93 with Ala in the S48T/F93A mutated enzyme, which resembles the, natural ... [(full description)]

About this Structure

1LDE is a [Single protein] structure of sequence from [Equus caballus] with ZN, NAD and FPI as [ligands]. Active as [[1]], with EC number [1.1.1.1]. Full crystallographic information is available from [OCA].

Reference

Binding of formamides to liver alcohol dehydrogenase., Ramaswamy S, Scholze M, Plapp BV, Biochemistry. 1997 Mar 25;36(12):3522-7. PMID:9132002

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