1euv
From Proteopedia
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X-RAY STRUCTURE OF THE C-TERMINAL ULP1 PROTEASE DOMAIN IN COMPLEX WITH SMT3, THE YEAST ORTHOLOG OF SUMO.
Overview
Modification of cellular proteins by the ubiquitin-like protein SUMO is, essential for nuclear processes and cell cycle progression in yeast. The, Ulp1 protease catalyzes two essential functions in the SUMO pathway: (1), processing of full-length SUMO to its mature form and (2) deconjugation of, SUMO from targeted proteins. Selective reduction of the proteolytic, reaction produced a covalent thiohemiacetal transition state complex, between a Ulp1 C-terminal fragment and its cellular substrate Smt3, the, yeast SUMO homolog. The Ulp1-Smt3 crystal structure and functional testing, of elements within the conserved interface elucidate determinants of SUMO, recognition, processing, and deconjugation. Genetic analysis guided by the, structure further reveals a regulatory element N-terminal to the, proteolytic domain that is required for cell growth in yeast.
About this Structure
1EUV is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast., Mossessova E, Lima CD, Mol Cell. 2000 May;5(5):865-76. PMID:10882122
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