1f1z
From Proteopedia
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TNSA, a catalytic component of the TN7 transposition system
Overview
Transposition requires a coordinated series of DNA breakage and joining, reactions. The Tn7 transposase contains two proteins: TnsA, which carries, out DNA breakage at the 5' ends of the transposon, and TnsB, which carries, out breakage and joining at the 3' ends of the transposon. TnsB is a, member of the retroviral integrase superfamily whose hallmark is a, conserved DDE motif. We report here the structure of TnsA at 2.4 A, resolution. Surprisingly, the TnsA fold is that of a type II restriction, endonuclease. Thus, Tn7 transposition involves a collaboration between, polypeptides, one containing a DDE motif and one that does not. This, result indicates that the range of biological processes that utilize, restriction enzyme-like folds also includes DNA transposition.
About this Structure
1F1Z is a Single protein structure of sequence from Escherichia coli with MG and CL as ligands. Full crystallographic information is available from OCA.
Reference
Unexpected structural diversity in DNA recombination: the restriction endonuclease connection., Hickman AB, Li Y, Mathew SV, May EW, Craig NL, Dyda F, Mol Cell. 2000 Jun;5(6):1025-34. PMID:10911996
Page seeded by OCA on Tue Nov 20 14:31:54 2007
