1f7y
From Proteopedia
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THE CRYSTAL STRUCTURE OF TWO UUCG LOOPS HIGHLIGHTS THE ROLE PLAYED BY 2'-HYDROXYL GROUPS IN ITS UNUSUAL STABILITY
Overview
All large structured RNAs contain hairpin motifs made of a stem closed by, several looped nucleotides. The most frequent loop motif is the UUCG one., This motif belongs to the tetraloop family and has the peculiarity of, being highly thermodynamically stable. Here, we report the first crystal, structure of two UUCG tetraloops embedded in a larger RNA-protein complex, solved at 2.8 A resolution. The two loops present in the asymmetric unit, are in a different crystal packing environment but, nevertheless, have an, identical conformation. The observed structure is globally close to that, obtained in solution by nuclear magnetic resonance. However, subtle, differences point to a more detailed picture of the role played by, 2'-hydroxyl groups in stabilising this tetraloop.
About this Structure
1F7Y is a Single protein structure of sequence from Thermus thermophilus with MG, NA and K as ligands. Full crystallographic information is available from OCA.
Reference
The crystal structure of UUCG tetraloop., Ennifar E, Nikulin A, Tishchenko S, Serganov A, Nevskaya N, Garber M, Ehresmann B, Ehresmann C, Nikonov S, Dumas P, J Mol Biol. 2000 Nov 17;304(1):35-42. PMID:11071808
Page seeded by OCA on Tue Nov 20 14:41:34 2007
Categories: Single protein | Thermus thermophilus | Dumas, P. | Ehresmann, B. | Ehresmann, C. | Ennifar, E. | Garber, M. | Nevskaya, N. | Nikonov, S. | Nikouline, A. | Serganov, A. | Tishchenko, S. | K | MG | NA | Ribosome | Rna | Tetraloop | Uucg | X-ray
