1fag
From Proteopedia
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STRUCTURE OF CYTOCHROME P450
Overview
The substrate-bound structures of two cytochrome P450s, P450cam and, P450eryF, are known. While these structures reveal important features that, control substrate specificity, the problem of how conformational changes, allow for substrate entry and product release remains unsolved. The, structure of the haem domain of the bacterial fatty acid hydroxylase, P450BM-3, previously was solved in the substrate-free form. Unlike the, substrate-bound P450cam and P450eryF structures, the substrate access, channel is open in substrate-free P450BM-3. Here we present the X-ray, structure of P450BM-3 at 2.7 A bound with a fatty acid substrate, palmitoleic acid. A comparison of the substrate-bound and -free forms, reveals major conformational differences and provides the first detailed, picture of substrate-induced conformational changes in a P450.
About this Structure
1FAG is a Single protein structure of sequence from Bacillus megaterium with HEM and PAM as ligands. Active as Unspecific monooxygenase, with EC number 1.14.14.1 Full crystallographic information is available from OCA.
Reference
The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid., Li H, Poulos TL, Nat Struct Biol. 1997 Feb;4(2):140-6. PMID:9033595
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