1fd2
From Proteopedia
|
SITE-DIRECTED MUTAGENESIS OF AZOTOBACTER VINELANDII FERREDOXIN I. (FE-S) CLUSTER-DRIVEN PROTEIN REARRANGEMENT
Overview
Azotobacter vinelandii ferredoxin I is a small protein that contains one, [4Fe-4S] cluster and one [3Fe-4S] cluster. Recently the x-ray crystal, structure has been redetermined and the fdxA gene, which encodes the, protein, has been cloned and sequenced. Here we report the site-directed, mutation of Cys-20, which is a ligand of the [4Fe-4S] cluster in the, native protein, to alanine and the characterization of the protein product, by x-ray crystallographic and spectroscopic methods. The data show that, the mutant protein again contains one [4Fe-4S] cluster and one [3Fe-4S], cluster. The new [4Fe-4S] cluster obtains its fourth ligand from Cys-24, a, free cysteine in the native structure. The formation of this [4Fe-4S], cluster drives rearrangement of the protein structure.
About this Structure
1FD2 is a Single protein structure of sequence from Azotobacter vinelandii with SF4 and F3S as ligands. Full crystallographic information is available from OCA.
Reference
Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: [Fe-S] cluster-driven protein rearrangement., Martin AE, Burgess BK, Stout CD, Cash VL, Dean DR, Jensen GM, Stephens PJ, Proc Natl Acad Sci U S A. 1990 Jan;87(2):598-602. PMID:2153958
Page seeded by OCA on Tue Nov 20 14:49:44 2007
