1fdn
From Proteopedia
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REFINED CRYSTAL STRUCTURE OF THE 2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDURICI AT 1.84 ANGSTROMS RESOLUTION
Overview
The crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium, acidurici has been determined at a resolution of 1.84 A and refined to an, R-factor of 0.169. Crystals belong to space group P4(3)2(1)2 with unit, cell dimensions a = b = 34.44 A and c = 74.78 A. The structure was, determined by molecular replacement using the previously published model, of an homologous ferredoxin and refined by molecular dynamics techniques., The model contains the protein and 46 water molecules. Only two amino acid, residues, Asp27 and Asp28, are poorly defined in the electron density, maps. The molecule has an overall chain fold similar to that of other, [4Fe-4S] bacterial ferredoxins of known structure. The two [4Fe-4S], clusters display similar bond distances and angles. In both of them the, co-ordination of one iron atom (bound to Cys11 and Cys40) is slightly, distorted as compared with that of the other iron atoms. A core of, hydrophobic residues and a few water molecules contribute to the stability, of the structure. The [4Fe-4S] clusters interact with the polypeptide, chain through eight hydrogen bonds each, in addition to the covalent, Fe-Scys bonds. The ferredoxin from Clostridium acidurici is the most, typical clostridial ferredoxin crystallized so far and the biological, implications of the newly determined structure are discussed.
About this Structure
1FDN is a Single protein structure of sequence from Clostridium acidurici with SF4 as ligand. Full crystallographic information is available from OCA.
Reference
Refined crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici at 1.84 A resolution., Duee ED, Fanchon E, Vicat J, Sieker LC, Meyer J, Moulis JM, J Mol Biol. 1994 Nov 4;243(4):683-95. PMID:7966291
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