1fmt
From Proteopedia
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METHIONYL-TRNAFMET FORMYLTRANSFERASE FROM ESCHERICHIA COLI
Overview
Formylation of the methionyl moiety esterified to the 3' end of tRNA(f)Met, is a key step in the targeting of initiator tRNA towards the translation, start machinery in prokaryotes. Accordingly, the presence of, methionyl-tRNA(f)Met formyltransferase (FMT), the enzyme responsible for, this formylation, is necessary for the normal growth of Escherichia coli., The present work describes the structure of crystalline E.coli FMT at 2.0, A, resolution. The protein has an N-terminal domain containing a Rossmann, fold. This domain closely resembles that of the glycinamide ribonucleotide, formyltransferase (GARF), an enzyme which, like FMT, uses N-10, formyltetrahydrofolate as formyl donor. However, FMT can be distinguished, from GARF by a flexible loop inserted within its Rossmann fold. In, addition, FMT possesses a C-terminal domain with a beta-barrel reminiscent, of an OB fold. This latter domain provides a positively charged side, oriented towards the active site. Biochemical evidence is presented for, the involvement of these two idiosyncratic regions (the flexible loop in, the N-terminal domain, and the C-terminal domain) in the binding of the, tRNA substrate.
About this Structure
1FMT is a Single protein structure of sequence from Escherichia coli. Active as Methionyl-tRNA formyltransferase, with EC number 2.1.2.9 Full crystallographic information is available from OCA.
Reference
Structure of crystalline Escherichia coli methionyl-tRNA(f)Met formyltransferase: comparison with glycinamide ribonucleotide formyltransferase., Schmitt E, Blanquet S, Mechulam Y, EMBO J. 1996 Sep 2;15(17):4749-58. PMID:8887566
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