6cmh
From Proteopedia
|
SYNTHETIC LINEAR MODIFIED ENDOTHELIN-1 AGONIST
Overview
The solution structure of a biologically active modified linear, endothelin-1 analogue, ET1-21[Cys(Acm)1,15, Aib3,11, Leu7], has been, determined for the first time by two-dimensional nuclear magnetic, resonance spectroscopy in a methanol-d3/water solvent mixture. Out of, approximately one hundred linear peptide analogues tested by biological, assay, this peptide, together with a dozen others, showed significant ETB, selective agonist activity. Here we report the solution structure of an, ETB selective agonist of a full-length, synthetic linear endothelin, analogue. The calculated structures indicate that the peptide adopts an, alpha-helical conformation between residues Ser5-His16, whilst both N- and, C-termini show no preferred conformation. These results suggest that the, disulphide bridges normally associated with endothelin and sarafotoxin, peptides may not necessarily be important for either ETB receptor binding, activity or the formation of a helical conformation in solution.
About this Structure
6CMH is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a novel ETB receptor selective agonist ET1-21 [Cys(Acm)1,15, Aib3,11, Leu7] by nuclear magnetic resonance spectroscopy and molecular modelling., Hewage CM, Jiang L, Parkinson JA, Ramage R, Sadler IH, J Pept Res. 1999 Mar;53(3):223-33. PMID:10231710
Page seeded by OCA on Tue Nov 20 15:30:45 2007
