1g1z

From Proteopedia

Revision as of 13:30, 20 November 2007 by OCA (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

1g1z

Drag the structure with the mouse to rotate

NMR Solution Structures of delta-Conotoxin EVIA from Conus ermineus that Selectively Acts on Vertebrate Neuronal Na+ Channels, LEU12-PRO13 Cis isomer

Overview

Delta-conotoxin EVIA, from Conus ermineus, is a 32-residue polypeptide, cross-linked by three disulfide bonds forming a four-loop framework., delta-Conotoxin EVIA is the first conotoxin known to inhibit sodium, channel inactivation in neuronal membranes from amphibians and mammals, (subtypes rNa(v)1.2a, rNa(v)1.3, and rNa(v)1.6), without affecting rat, skeletal muscle (subtype rNa(v)1.4) and human cardiac muscle (subtype, hNa(v)1.5) sodium channel (Barbier, J., Lamthanh, H., Le Gall, F., Favreau, P., Benoit, E., Chen, H., Gilles, N., Ilan, N., Heinemann, S. F., Gordon, D., Menez, A., and Molgo, J. (2004) J. Biol. Chem. 279, 4680-4685). Its structure was solved by NMR and is characterized by a 1:1, cis/trans isomerism of the Leu(12)-Pro(13) peptide bond in slow exchange, on the NMR time scale. The structure of both cis and trans isomers could, be calculated separately. The isomerism occurs within a specific long, disordered loop 2, including residues 11-19. These contribute to an, important hydrophobic patch on the surface of the toxin. The rest of the, structure matches the "inhibitor cystine-knot motif" of conotoxins from, the "O superfamily" with a high structural order. To probe a possible, functional role of the Leu(12)-Pro(13) cis/trans isomerism, a Pro(13) -->, Ala delta-conotoxin EVIA was synthesized and shown to exist only as a, trans isomer. P13A delta-conotoxin EVIA was estimated only two times less, active than the wild-type EVIA in binding competition to rat brain, synaptosomes and when injected intracerebroventricularly into mice.

About this Structure

1G1Z is a Single protein structure of sequence from [1] with NH2 as ligand. Full crystallographic information is available from OCA.

Reference

NMR solution structures of delta-conotoxin EVIA from Conus ermineus that selectively acts on vertebrate neuronal Na+ channels., Volpon L, Lamthanh H, Barbier J, Gilles N, Molgo J, Menez A, Lancelin JM, J Biol Chem. 2004 May 14;279(20):21356-66. Epub 2004 Feb 19. PMID:14976206

Page seeded by OCA on Tue Nov 20 15:38:06 2007

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools