1g5g

From Proteopedia

FRAGMENT OF FUSION PROTEIN FROM NEWCASTLE DISEASE VIRUS

Overview

BACKGROUND: Membrane fusion within the Paramyxoviridae family of viruses, is mediated by a surface glycoprotein termed the "F", or fusion, protein., Membrane fusion is assumed to involve a series of structural transitions, of F from a metastable (prefusion) state to a highly stable (postfusion), state. No detail is available at the atomic level regarding the metastable, form of these proteins or regarding the transitions accompanying fusion., RESULTS: The three-dimensional structure of the fusion protein of, Newcastle disease virus (NDV-F) has been determined. The trimeric NDV-F, molecule is organized into head, neck, and stalk regions. The head is, comprised of a highly twisted beta domain and an additional, immunoglobulin-like beta domain. The neck is formed by the C-terminal, extension of the heptad repeat region HR-A, capped by a four-helical, bundle. The C terminus of HR-A is encased by a further helix HR-C and a, 4-stranded beta sheet. The stalk is formed by the remaining visible, portion of HR-A and by polypeptide immediately N-terminal to the, C-terminal heptad repeat region HR-B. An axial channel extends through the, head and neck and is fenestrated by three large radial channels located, approximately at the head-neck interface. CONCLUSION: We propose that, prior to fusion activation, the hydrophobic fusion peptides in NDV-F are, sequestered within the radial channels within the head, with the central, HR-A coiled coil being only partly formed. Fusion activation then, involves, inter alia, the assembly of a complete HR-A coiled coil, with, the fusion peptides and transmembrane anchors being brought into close, proximity. The structure of NDV-F is fundamentally different than that of, influenza virus hemagglutinin, in that the central coiled coil is in the, opposite orientation with respect to the viral membrane.

About this Structure

1G5G is a Single protein structure of sequence from Newcastle disease virus with NAG as ligand. Full crystallographic information is available from OCA.

Reference

The structure of the fusion glycoprotein of Newcastle disease virus suggests a novel paradigm for the molecular mechanism of membrane fusion., Chen L, Gorman JJ, McKimm-Breschkin J, Lawrence LJ, Tulloch PA, Smith BJ, Colman PM, Lawrence MC, Structure. 2001 Mar 7;9(3):255-66. PMID:11286892

Page seeded by OCA on Tue Nov 20 15:43:19 2007