This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1gxp
From Proteopedia
|
PHOB EFFECTOR DOMAIN IN COMPLEX WITH PHO BOX DNA.
Overview
PhoB is a signal transduction response regulator that activates nearly 40, genes in phosphate depletion conditions in E. coli and closely related, bacteria. The structure of the PhoB effector domain in complex with its, target DNA sequence, or pho box, reveals a novel tandem arrangement in, which several monomers bind head to tail to successive 11-base pair, direct-repeat sequences, coating one face of a smoothly bent double helix., The protein has a winged helix fold in which the DNA recognition elements, comprise helix alpha 3, penetrating the major groove, and a beta hairpin, wing interacting with a compressed minor groove via Arg219, tightly, sandwiched between the DNA sugar backbones. The transactivation loops, protrude laterally in an appropriate orientation to interact with the RNA, polymerase sigma(70) subunit, which triggers transcription initiation.
About this Structure
1GXP is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator., Blanco AG, Sola M, Gomis-Ruth FX, Coll M, Structure. 2002 May;10(5):701-13. PMID:12015152
Page seeded by OCA on Tue Nov 20 16:20:11 2007
