1haa
From Proteopedia
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A BETA-HAIRPIN STRUCTURE IN A 13-MER PEPTIDE THAT BINDS A-BUNGAROTOXIN WITH HIGH AFFINITY AND NEUTRALIZES ITS TOXICITY
Overview
Snake-venom alpha-bungarotoxin is a member of the alpha-neurotoxin family, that binds with very high affinity to the nicotinic acetylcholine receptor, (AChR) at the neuromuscular junction. The structure of the complex between, alpha-bungarotoxin and a 13-mer peptide (WRYYESSLEPYPD) that binds the, toxin with high affinity, thus inhibiting its interactions with AChR with, an IC(50) of 2 nM, has been solved by (1)H-NMR spectroscopy. The bound, peptide folds into a beta-hairpin structure created by two antiparallel, beta-strands, which combine with the already existing triple-stranded, beta-sheet of the toxin to form a five-stranded intermolecular, antiparallel beta-sheet. Peptide residues Y3(P), E5(P), and L8(P) have the, highest intermolecular contact area, indicating their importance in the, binding of alpha-bungarotoxin; W1(P), R2(P), and Y4(P) also contribute, significantly to the binding. A large number of characteristic hydrogen, bonds and electrostatic and hydrophobic interactions are observed in the, complex. The high-affinity peptide exhibits inhibitory potency that is, better than any known peptide derived from AChR, and is equal to that of, the whole alpha-subunit of AChR. The high degree of sequence similarity, between the peptide and various types of AChRs implies that the binding, mode found within the complex might possibly mimic the receptor binding to, the toxin. The design of the high-affinity peptide was based on our, previous findings: (i) the detection of a lead peptide (MRYYESSLKSYPD), that binds alpha-bungarotoxin, using a phage-display peptide library, (ii), the information about the three-dimensional structure of, alpha-bungarotoxin/lead-peptide complex, and (iii) the amino acid sequence, analysis of different AChRs.
About this Structure
1HAA is a Protein complex structure of sequences from Bungarus multicinctus. Full crystallographic information is available from OCA.
Reference
A beta -hairpin structure in a 13-mer peptide that binds alpha -bungarotoxin with high affinity and neutralizes its toxicity., Scherf T, Kasher R, Balass M, Fridkin M, Fuchs S, Katchalski-Katzir E, Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6629-34. Epub 2001 May 29. PMID:11381118
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