1hw2
From Proteopedia
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FADR-DNA COMPLEX: TRANSCRIPTIONAL CONTROL OF FATTY ACID METABOLISM IN ECHERICHIA COLI
Overview
In Escherichia coli, the expression of fatty acid metabolic genes is, controlled by the transcription factor, FadR. The affinity of FadR for DNA, is controlled by long chain acyl-CoA molecules, which bind to the protein, and modulate gene expression. The crystal structure of FadR reveals a two, domain dimeric molecule where the N-terminal domains bind DNA, and the, C-terminal domains bind acyl-CoA. The DNA binding domain has a, winged-helix motif, and the C-terminal domain resembles the sensor domain, of the Tet repressor. The FadR.DNA complex reveals how the protein, interacts with DNA and specifically recognizes a palindromic sequence., Structural and functional similarities to the Tet repressor and the BmrR, transcription factors suggest how the binding of the acyl-CoA effector, molecule to the C-terminal domain may affect the DNA binding affinity of, the N-terminal domain. We suggest that the binding of acyl-CoA disrupts a, buried network of charged and polar residues in the C-terminal domain, and, the resulting conformational change is transmitted to the N-terminal, domain via a domain-spanning alpha-helix.
About this Structure
1HW2 is a Single protein structure of sequence from Escherichia coli with MG as ligand. Full crystallographic information is available from OCA.
Reference
The FadR.DNA complex. Transcriptional control of fatty acid metabolism in Escherichia coli., Xu Y, Heath RJ, Li Z, Rock CO, White SW, J Biol Chem. 2001 May 18;276(20):17373-9. Epub 2001 Feb 13. PMID:11279025
Page seeded by OCA on Tue Nov 20 16:51:47 2007
