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1ij5
From Proteopedia
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METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD
Overview
Acellular slime mold, Physarum polycephalum, has a unique wound-healing, system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large, aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N, terminus by a proteinase in plasmodia (CBP40delta), which does not, aggregate in the Ca(2+)-bound form. Here we report the crystal structures, of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both, structures consist of three domains: coiled-coil, intervening, and, EF-hand. The topology of the EF-hand domain is similar to that of calpain., The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands, through a large hydrophobic interface, necessary for high Ca(2+) affinity., Conformational change upon Ca(2+) binding is small; however, the structure, of CBP40delta provides novel insights into the mechanism of, Ca(2+)-dependent oligomerization.
About this Structure
1IJ5 is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
Metal-free and Ca2+-bound structures of a multidomain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum., Iwasaki W, Sasaki H, Nakamura A, Kohama K, Tanokura M, Structure. 2003 Jan;11(1):75-85. PMID:12517342
Page seeded by OCA on Tue Nov 20 17:26:28 2007
