This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1irx
From Proteopedia
|
Crystal structure of class I lysyl-tRNA synthetase
Overview
Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II, (LysRS-II) lysyl-tRNA synthetase. The crystal structure of LysRS-I from, Pyrococcus horikoshii at 2.6 A resolution reveals extensive similarity, with glutamyl-tRNA synthetase (GluRS). A comparison of the structures of, LysRS-I and LysRS-II in complex with lysine shows that both enzymes use, similar strategies for substrate recognition within unrelated active site, topologies. A docking model based upon the GluRS-tRNA complex suggests how, LysRS-I and LysRS-II can recognize the same molecular determinants in, tRNALys, as shown by biochemical results, while approaching the acceptor, helix of the tRNA from opposite sides.
About this Structure
1IRX is a Single protein structure of sequence from Pyrococcus horikoshii with ZN as ligand. Active as Lysine--tRNA ligase, with EC number 6.1.1.6 Full crystallographic information is available from OCA.
Reference
Functional convergence of two lysyl-tRNA synthetases with unrelated topologies., Terada T, Nureki O, Ishitani R, Ambrogelly A, Ibba M, Soll D, Yokoyama S, Nat Struct Biol. 2002 Apr;9(4):257-62. PMID:11887185
Page seeded by OCA on Tue Nov 20 17:37:03 2007
Categories: Lysine--tRNA ligase | Pyrococcus horikoshii | Single protein | Ambrogelly, A. | Ibba, M. | Ishitani, R. | Nureki, O. | RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative. | Soll, D. | Terada, T. | Yokoyama, S. | ZN | Alpha-helix cage | Beta sandwitch | Riken structural genomics/proteomics initiative | Rossmann fold | Rsgi | Structural genomics | Zinc-binding structure
