1is7
From Proteopedia
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Crystal structure of rat GTPCHI/GFRP stimulatory complex
Overview
In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory, protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase, I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of, tetrahydrobiopterin. The crystal structure of the stimulatory complex, reveals that the GTPCHI decamer is sandwiched by two GFRP homopentamers., Each GFRP pentamer forms a symmetrical five-membered ring similar to, beta-propeller. Five phenylalanine molecules are buried inside each, interface between GFRP and GTPCHI, thus enhancing the binding of these, proteins. The complex structure suggests that phenylalanine-induced GTPCHI, x GFRP complex formation enhances GTPCHI activity by locking the enzyme in, the active state.
About this Structure
1IS7 is a Protein complex structure of sequences from Rattus norvegicus with K and PHE as ligands. Active as GTP cyclohydrolase I, with EC number 3.5.4.16 Full crystallographic information is available from OCA.
Reference
Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP., Maita N, Okada K, Hatakeyama K, Hakoshima T, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1212-7. Epub 2002 Jan 29. PMID:11818540
Page seeded by OCA on Tue Nov 20 17:37:47 2007
