1ig5
From Proteopedia
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BOVINE CALBINDIN D9K BINDING MG2+
Overview
The three-dimensional structures of the magnesium- and manganese-bound, forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly, identical but deviate significantly from both the calcium bound form and, the metal ion-free (apo) form. The largest structural differences are seen, in the C-terminal EF-hand, and involve changes in both metal ion, coordination and helix packing. The N-terminal calcium binding site is not, occupied by any metal ion in the magnesium and manganese structures, and, shows little structural deviation from the apo and calcium bound forms., 1H-NMR and UV spectroscopic studies at physiological ion concentrations, show that the C-terminal site of the protein is significantly ... [(full description)]
About this Structure
1IG5 is a [Single protein] structure of sequence from [Bos taurus] with MG as [ligand]. This structure superseeds the now removed PDB entry 5ICB. Full crystallographic information is available from [OCA].
Reference
Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k., Andersson M, Malmendal A, Linse S, Ivarsson I, Forsen S, Svensson LA, Protein Sci. 1997 Jun;6(6):1139-47. PMID:9194174
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