1j4j
From Proteopedia
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Crystal Structure of Tabtoxin Resistance Protein (form II) complexed with an Acyl Coenzyme A
Overview
Tabtoxin resistance protein (TTR) is an enzyme that renders, tabtoxin-producing pathogens, such as Pseudomonas syringae, tolerant to, their own phytotoxins. Here, we report the crystal structure of TTR, complexed with its natural cofactor, acetyl coenzyme A (AcCoA), to 1.55A, resolution. The binary complex forms a characteristic "V" shape for, substrate binding and contains the four motifs conserved in the, GCN5-related N-acetyltransferase (GNAT) superfamily, which also includes, the histone acetyltransferases (HATs). A single-step mechanism is proposed, to explain the function of three conserved residues, Glu92, Asp130 and, Tyr141, in catalyzing the acetyl group transfer to its substrate. We also, report that TTR possesses HAT activity and suggest an evolutionary, relationship between TTR and other GNAT members.
About this Structure
1J4J is a Single protein structure of sequence from Pseudomonas syringae pv. tabaci with ACO as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of tabtoxin resistance protein complexed with acetyl coenzyme A reveals the mechanism for beta-lactam acetylation., He H, Ding Y, Bartlam M, Sun F, Le Y, Qin X, Tang H, Zhang R, Joachimiak A, Liu J, Zhao N, Rao Z, J Mol Biol. 2003 Jan 31;325(5):1019-30. PMID:12527305
Page seeded by OCA on Tue Nov 20 17:55:48 2007
Categories: Pseudomonas syringae pv. tabaci | Single protein | Bartlam, M. | Cao, Z. | Ding, Y. | Duke, N. | He, H. | Jiang, F. | Joachimiak, A. | Liu, J. | Liu, Y. | Rao, Z. | Shao, Y. | Tang, H. | Zhang, R. | Zhao, N. | ACO | Transferase
